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Azaspiracid accumulation, detoxification and biotransformation in blue mussels (Mytilus edulis) experimentally fed Azadinium spinosum ArchiMer
Jauffrais, Thierry; Marcaillou-le Baut, Claire; Herrenknecht, Christine; Truquet, Philippe; Sechet, Veronique; Nicolau, Elodie; Tillmann, Urban; Hess, Philipp.
Azadinium spinosum (Elbrächter and Tillmann), a small marine dinoflagellate, has been recently described as a de novo producer of azaspiracid-1 and -2 (AZA1 and -2) diarrhoeic toxins. A culture of A. spinosum was established in our laboratory and optimised for pilot-scale production of this organism, to evaluate and understand AZA1 and -2 accumulation and biotransformation in blue mussels (Mytilus edulis) fed with A. spinosum. Adult mussels were continuously exposed to A. spinosum over 1 week in 160 L cylindrical conical tanks. Three different diets were tested for contamination: 5000, 10 000 cells mL−1 of A. spinosum and a mixture of 5000 cells mL−1 of A. spinosum with 5000 cells mL−1 of Isochrysis aff. galbana (T-Iso, CCAP 927/14). During the subsequent...
Tipo: Text Palavras-chave: Azaspiracid; Azadinium spinosum; Marine biotoxins; AZA; Tissue distribution; Histology; Bivalve molluscs; Liquid chromatography coupled to tandem mass spectrometry.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00088/19877/17566.pdf
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COLNACOQ. Composés lipophiles naturels dans l’environnement des coquillages. Rapport final ArchiMer
Hess, Philipp; Geiger, Marie; Brochard, Solène; Lepretre, Thomas; Fessard, Valerie; Antignac, Jean-philippe; Dupont, Jacques; Sechet, Veronique; Vanel, Faustine; Mondeguer, Florence; Herve, Fabienne; Leborgne, Sabrina; Deslanglois, Gwenaëlle; Marshall, Lindsey; Rounds, Lucy; Guitton, Yann; Amzil, Zouher; Grovel, Olivier; Ruiz, Nicolas; Pouchus, Francois.
Cette étude s’est inscrite dans un contexte d’évaluation de la salubrité des mollusques bivalves destinés à la consommation humaine. Ces organismes peuvent en effet accumuler des toxines, en particulier de microalgues, en concentrations importantes, pouvant induire des intoxications chez les personnes les consommant. Afin d’assurer la protection des consommateurs, les toxines réglementées sont recherchées dans les coquillages par techniques physico-chimiques. En parallèle, les toxines émergentes, potentiellement produites par d’autres organismes que les micro-algues telles que les micromycètes, peuvent être detectées par l’utilisation du test de toxicité aigüe sur souris. Cependant, ce bio-essai présente de très nombreux inconvénients, notamment éthiques...
Tipo: Text Palavras-chave: Cytotoxicité; Toxicité sur larves de diptères; Activité antibactérienne; Bactéries marines; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00253/36470/35015.pdf
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Écophysiologie des dinoflagellés du genre Azadinium, production toxinique et transfert trophique vers les mollusques bivalves ArchiMer
Jauffrais, Thierry.
This study has been conducted in order to develop the analysis of AZAs and to produce AZAs from A. spinosum culture. It also aimed at studying the effect of environmental and nutritional factors on growth and toxin production. The study also demonstrated a link between A. spinosum and the accumulation of AZAs in shellfish, followed by the clarification of the processes of accumulation, detoxification and biotransformation of AZAs into mussels. A quantitative analysis of AZAs in A. spinosum cultures was developed, and the formation and structure of the AZA methylated analogues was explained and minimised. This work also demonstrated the feasibility of a sustainable production of AZAs from A. spinosum culture and highlighted the main factors influencing...
Tipo: Text Palavras-chave: Azadinium spinosum; Mytilus edulis; Azaspiracides; Phycotoxines; Dinoflagellés; Écophysiologie; CL-SM/SM; Azadinium spinosum; Mytilus edulis; Azaspiracids; Phycotoxins; Dinoflagellate; Ecophysiology; LC-MS/MS.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00111/22217/19891.pdf
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Évaluation d’une suite de bio-essais pour la détection et l’étude de composés lipophiles de micro-organismes marins issus de mollusques bivalves et de leur environnement ArchiMer
Geiger, Marie.
The context of this study was the assessment of the safety of edible bivalve molluscs. Bivalve can indeed accumulate high concentrations of phycotoxins into their hepatopancreas, potentially leading to human intoxication outbreaks after consumption of such contaminated bivalves. In order to ensure the protection of shellfish consumers, regulated toxins are monitored using physico-chemical techniques. In parallel, emerging toxins, that can be putatively produced by microalgae or marine fungi, are monitored using the intraperitoneal mouse bioassay. However, this bioassay suffers ethical and methodological drawbacks. Thus, the objective of this work was to assess the ability of a bioassay suite to detect lipophilic toxins. This suite was composed of three...
Tipo: Text Palavras-chave: Cytotoxicité; Larves de diptères; Activité antibactérienne; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii; Cytotoxicity; Diptera larvae; Antibacterial activity; Lipophilic toxins; Microalgae; Dinoflagellates; Marine fungi; Penicillium; Okadaic acid; Azaspiracid; Pinnatoxin G; Vulcanodinium rugosum; Azadinium spinosum; Prorocentrum lima; Beauveria brongniartii.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00183/29426/27787.pdf
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